DOI: 10.36347/sjams.2017.v05i01.045

Pages: 232-236

Various enzymes has evaluated by analyzing pleural fluid to diagnose Tubercular pleural effusion. Among different enzymes, Adenosine deaminase has been proven with higher specificity and sensitivity, to be a good marker for tuberculosis. The aim of this study is to evaluate the utility of Adenosine deaminase in serum and pleural fluids in the diagnosis of HIV seronegative Tuberculous pleural effusion. A total of 120 patients with Exudative pleural effusion of known etiology with HIV seronegative status were selected to do this study. Out of 120 patients, 60 were with new cases of Tuberculous pleural effusion and those were considered as cases. Remaining 60 patients were with non Tuberculous pleural effusion, were considered as controls. Serum or Plasma and Pleural fluid samples were collected under aseptic precautions from selected groups and sent for investigation. After collection samples were analyzed for Adenosine deaminase by cross checking in Systronics UV spectrophotometer, spectrophotometer and Transasia semi auto analyzer with precinorm and precipath in duplicates. ADA was analyzed by Guisti G and Galanti Method. The mean value of ADA in serum samples of among tuberculous and non tuberculous pleural effusion patients was 75.12±2.83 and 17.43±3.62 respectively. The Mean value of ADA levels in pleural fluid among tuberculous and non tuberculous pleural effusion patients was 86.42±3.48 and 15.48±5.23 respectively. On analyzing the statistical significance of ADA levels between tuberculous and non tuberculous pleural effusion, shown that it was extremely statistically significant. ADA test have a higher degree of specificity and sensitivity, which is a simple, quick, less expensive and superior diagnostic test for exudative pleural effusion. ADA testing requires just a simple colorimeter.