DOI: 10.36347/sjams.2017.v05i02.036

Pages: 487-494

This study aimed to determine the frequency of extended spectrum beta lactamase producing strains among gram negative bacilli causing nosocomial infections. In addition to determine the drug resistance pattern of using pathogens isolated from nosocomial patients using Kirby-Bauer disc diffusion method. The present study was a descriptive cross-sectional study conducted between September 2011 to September 2012 from hospitalized patients suspected of having Gram negative diseases at Port Sudan teaching hospital in red sea state. Isolation and identification of pathogenic bacteria were carried out following standard laboratory procedures. All isolates were tested to 14 types of common antimicrobial uses. Identification of extended spectrum beta lactamase (ESBL) production was performed by the double disk synergy test and double disk diffusion test. Collected data were analyzed by using Statistical Package for Social Sciences (SPSS; Version 20). P value <0.05 were considered statistically significant. Accordingly, our results showed 198 types of Gram negative bacteria isolated. The major isolates was E.coli (89/44.9%) and the least Gram negative organism isolated were Klebsiella oxytoca, Serratia marcescens, Morganella morganii, Salmonella para typhi A and Citrobacter koseri (1/0.5%). This difference was statistically significant (p 0.00). ESBL producing bacteria was 44.4%, and was mostly E.coli 63.6%. The maximum sensitivity was seen for amikacin (96.6%) the different was statistically significant (p 0.00). While the maximum resistance was seen against Ceftriaxone, Ciprofloxacin and Tetracycline (100%).