DOI: 10.36347/sjet.2022.v10i10.003

Pages: 279-284

The purpose of this study is to assess the Physicochemical properties such as (Specific Gravity, Refractive Index, Acid Value, Percentage Free Fatty Acids, Peroxide Value, Saponification Value and Iodine Value), investigate the chemical constituents of the Fixed Oil from Brassica Juncea L seeds and to evaluate its potential antibacterial activity against five microorganisms (Bacillus Subitus, Staphylococcus aureus, Escherichia Coli, Salmonella, Penicillium and Aspergillus Niger). The chemical constituents of Brassica Juncea L seeds oil were identified and quantified by GC-MS, where disc diffusion assay were employed to evaluate the antibacterial activities and physicochemical properties using standard methods. Eight components that have been identified detected revealed the following major components: 13-Docosenoic Acid, Methyl Ester, (Z) (59.02%), 9,12-Octadecadienoic Acid, Methyl Ester (14.65%),10-Octadecenoic Acid, Methyl Ester (8.66%) and cis-11-Eicosenoic Acid, Methyl Ester (7.82%). The physicochemical properties of Brassica Juncea L seed oil showed (Specific Gravity (0.923), Refractive Index (1.469), Acid Value (7.62), Percentage Free Fatty Acids (1.1), Peroxide Value (12.4), Saponification Value (18.23) and Iodine Value (110.35). The antibacterial activity showed partial activity against both Gram-positive and Gram-negative bacteria and inactive against the fungi. In conclusion, the results showed that the Oil of Brassica Juncea L Seeds is a potential source of natural antibacterial, and justify its uses in folkloric medicines.