DOI: 10.36347/sjams.2015.v03i03.011

Pages: 1092-1095

The transition from mould to yeast form in a standard strain of Sporothrix schenckii (ATCC 14284) was attained by sub-culturing mycelia or conidia on enriched culture medium, blood agar (BA). Five successive subcultures were done to obtain yeast form of S. schenckii. A growth curve was determined using transformed standard strain of S. schenckii from mycelial to yeast form. Yeast nitrogen base medium (YNB) was used to study the different phases of growth in the yeast form of S. schenckii. After doing the sub-culture, the optical density (OD) was recorded and then tube was incubated at 37ºC, thereafter, daily readings of OD were taken to obtain a growth curve. Lag phase was observed from time of inoculation to day one. Log phase was observed from day 2 to day 18 followed by the stationary phase till day 24. Thereafter, an unceasing decline in ODs was observed till day 34 and considered as the death phase.