DOI: 10.36347/sjds.2016.v03i08.001

Pages: 215-219

Abstract: Advancement of regenerative treatments using stem cells, materials used in regenerative treatment could have be have the ability to stimulate hard tissue formation and positive effect on osteogenesis and dentinogenesis differentiation of stem cells. Stem cells from apical papilla (SCAPs) are one of the mesenchymal stem cells derived from the embryonic neural crest, with greater odonto/osteogenic capacity and outstanding dentinogenesis. Therefore in this study we evaluated the effect of Portland cement and on odontogenic and osteogenic differentiation of this cells. Multiple colony-derived stem cells were isolated from the apical papillae, and the effects of material on the differentiation of SCAPs were investigated in vitro by using ALP activity measure, alizarin red staining and Real Time PCR. Alizarin red and alkaline phosphatase staining showed mineralization in Portland group. The results of the evaluation of gene expression showed that the majority of the studied genes associated with the differentiation of osteogenic and odontogenic in Portland group increased significantly compared to the control group. (P < 0.001). Portland cement have the ability to induce mineralization process and stimulate osteogenic and odontogenic differentiation.