DOI: 10.36347/sjams.2015.v03i06.050

Pages: 2393-2396

Bacterial vaginosis (BV) is the most common cause of vaginal discharge among women of childbearing age. .Its prevalence varies from 10 to 65% and is higher in sexually transmitted disease clinics. This study was carried out to compare the laboratory investigative methods with clinical assessment criteria for diagnosis of bacterial vaginosis. This prospective study included 200 vaginal swabs complaining of abnormal vaginal discharge. Amsels criteria, wet mount preparation and smears for Gram staining were prepared for Nugent criteria.5Anaerobic cultures were done for isolation of anaerobic bacteria associated with BV. In results Total 128 patients showed bacterial vaginosis according to the amsel’s criteria. Gram staining for Nugent scoring was examined by two different evaluators to see inter evaluator variations. The inter observer evaluation complete agreement was shown by 90.5 % i.e. in the 181 slides with agreement on slides, both the investigators scored the same group normal, intermediate or BV. While the interpretation of slides were in disagreement only in 9.5% of cases that too between intermediate and BV group. There is no disagreement in negative cases between both the observers. So interrater reproducibility was excellent between both the observers Out of 200 cases, 176 were culture positive for anaerobes other than lactobacilli. The Gram negative anaerobes out-numbered the Gram positive ones. In conclusion the prevalence of BV in developing countries are high So with limited resources countries such as India, diagnosis of BV , there is a great need for inexpensive diagnostic methods for bacterial vaginosis.