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Scholars Journal of Applied Medical Sciences | Volume-3 | Issue-07
Effect of Pipette Calibration on Quality Control Results in Khartoum State 2014
Fatima alzahraa Eldaw Ibrahim, Nassr Eldin M.A. Shrif
Published: Oct. 25, 2015 | 101 68
DOI: 10.36347/sjams.2015.v03i07.003
Pages: 2457-2460
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Abstract
Pipette calibration significantly impacts the quality of liquid delivery in the clinical laboratory ,the aim of this study to determine that the effect of automatic pipette calibration on quality control results ,the experimental study involved 10 automatic pipettes 5 pipettes are with volume 1.0 ml and another 5 are with volume 0.1 ml ,all pipettes were examined for work calibration the precision and accuracy of automatic pipettes were estimated by using DW weighting method recommended by WHO according to ISO 8655.DIN 12650 after that precision and accuracy for urea, uric acid, and glucose were estimated in normal control sera by using CLSI document EP05.A2 describe the procedure for determining the precision pre and post calibration, the study found that a high percentage of pipettes performed outside. Established specifications for precision, accuracy and need recalibration .inaccuracy and imprecision of automatic pipettes had high effect on results of normal control the most important aspect of pipette quality control is a calibration and all pipettes must be periodically checked and also all new automatic pipettes must be verified and recalibrated.