DOI: 10.36347/sjams.2015.v03i07.046

Pages: 2676-2679

This study describes the in vitro effect of potassium iodide (KI) on the growth of filamentous form of Sporothrix schenckii (S. schenckii) in broth by turbidity method. A growth curve was determined using a standard strain of S. schenckii (ATCC 14284). Yeast nitrogen base medium (YNB) was used to study the different phases of growth in the mycelial form of S. schenckii. After doing the sub-culture, the optical density (OD) was recorded and then tube was incubated at 25ºC, thereafter, daily readings of OD were taken to obtain a growth curve upto 34 days. After knowing the normal growth pattern of S. schenckii, the effect of increasing concentrations of KI on growth of S. schenckii (mycelial phase) was observed. YNB medium was prepared and potassium iodide was added into the medium in increasing concentrations in such a way so as to have final concentrations of 0.05, 0.1, 0.2, 0.4, 0.8, 1.6, 3.2, 6.4 and 12.8 gram percent of the medium. These tubes were inoculated with standard inoculum and incubated at 25ºC upto 34 days. Daily record of growth was maintained by recording the optical density of the tubes. With increasing KI concentration a significant decrease in optical densities as compared to control were observed (F=16.198; p<0.001). It reveals that KI has inhibitory effect on the growth of S. schenckii.