DOI: 10.36347/sjams.2015.v03i07.055

Pages: 2713-2718

The choice of antibiotics to treat Pseudomonas infections of eye is challenging to ophthalmologist, considering the increase in prevalence of antibiotic resistant isolates. With the increase in occurrence and types of multiple β- lactamase enzymes, early detection is crucial, the benefits of which include implementation of proper antibiotic therapy and infection control policy. The present study was conducted for the isolation, identification of Pseudomonas species & its antibiotic susceptibility patterns in ocular infections with special reference to ESBL, MBL, and AMP-C beta lactamase detection. The present study was carried out at Department of Microbiology, Sarojini Devi Eye Hospital, Hyderabad, from March 2014 to August 2014. ESBL detection was done by combined disc test (CDT) & double disc synergy test (DDST). MBL detection was done by Ceftazidime-EDTA CDT and Ceftazidime-EDTA DDST. Amp-C detection was done by Disc antagonism test &AmpC disc test. Out of the 20 Pseudomonas isolated, 4(20%) were ESBL producers, 10(50%) were MBL producers, and 1 isolate (5%) was AmpC producer. Most sensitive antibiotic was Cefotaxime (100%), Amikacin (60%) followed by Ciprofloxacin &Gatifloxacin (50%) each. The present study emphasizes high prevalence of Pseudomonas producing beta-lactamase enzymes, creating therapeutic challenge for Clinicians and Microbiologists. Hence, routine surveillance of antibiotic resistance is required in the hospital.