DOI: 10.36347/sajb.2013.v01i06.006

Pages: 252-262

The present study aims to determine the occurrence of ochratoxigenic fungi and ochratoxin A in Yemeni green coffee (Coffea arabica L.), by analyzing 70 samples which were collected from many local markets in some Yemeni Governorates during 2010/2011. The analysis carried out in Natural Products Chemistry Laboratories, Indian Institute of Chemical Technology (I.I.C.T), Hyderabad, India, and the results could be summarized as the following: by classification of Aspergillus genus we found these species Aspergillus niger, Aspergillus ochraceus, Aspergillus fumigatus, Aspergillus flavus, Aspergillus parasiticus, Aspergillus acceleatus and Aspergillus candidus by percentages were 27.5, 17.5, 15, 12.5, 12.5, 10 and 5%, respectively. And when we determine their (toxicity) ability to produced ochratoxin A in (YES) medium we found these percentages 30, 40, 20, 20, 0, 0 and 0 %, respectively. Meanwhile, the concentrations of ochratoxin A were ranged between 0.152 to 12.456 ng/ml of (YES) medium for 15 days growth. On the other hand, the positive samples of Yemeni green coffee beans which contaminated by ochratoxin A were 7 out of 70 samples by percentage of 10%, and the concentration of toxin ranged between 0.314 to 3.443 ng/g and the average was 1.211 ng ochratoxin A / per g of Yemeni green coffee beans.