DOI: 10.36347/sajb.2013.v01i06.009

Pages: 277-282

Forty five wound specimens were collected from patients suffering from wound infections and taken from various hospitals in Ibb city, Yemen. The study was to determine synergic antibacterial activity of between mountain honey and Argemone mexicana plant. Isolation, identification of bacterial isolates and antibiotic sensitivity test were done. Agar-disc and agar-well diffusion method were carried to determine antibacterial activity of honey, Argemone mexicana plant and a mixture of them against bacterial isolates. Out of 45 specimens, 29 (64.4%) gave positive cultures. Staphylococcus aureus was the predominant bacterial pathogens with percentage (72.4%) followed by Pseudomonas aeruginosa (17.2%) and Staphylococcus epidermidis (10.4%). The highest concentration (70%) of honey was more effective than other concentrations and crude milky sap of A.mexicana plant had higher antibacterial activity against pathogenic bacteria than any milky sap dilutions. The mixture of honey and A.mexicana plant gave an excellent inhibitor effect against bacterial growth. Mixture of honey (70%) and crude milky sap of A.mexicana revealed higher antibacterial activity against pathogenic bacteria comparing with honey and plant crude extract alone.