DOI: 10.36347/sajb.2014.v02i03.005

Pages: 193-201

Phenol and its compounds are one of the most important pollutants in the environment. P-Nitrophenol (PNP) is a toxic compound that enters the environment during manufacturing and processing of a variety of industrial products. In this context, the study was conducted on biodegradation of phenolic compound; PNP by P. putida MTCC 1194. The present study was also examined the efficiency of biodegradation of PNP on different pH. When the ph was increased up to 9, transformed organism possessed nullified effect towards the 72hrs of all the treated concentrations. Though, in wild type colonies had a remarkably significant (p<0.0029) biodegrading efficiency noted in each different concentrations with its stipulated period of time. Protein was isolated from P. putida and transformed E. coli they were run on SDS-PAGE and molecular weight was determined that P. putida have lower molecular weighed protein band such as 45.5 KDa, and transformed E. coli contains higher molecular weighed protein 62 KDa the GCMS spectral analysis for P. putida, It will showed that totally seven compounds has been identified. Amoung the seven compounds two peak compounds also analysed such as 5, 6-dibromo-N, N-dimethyltryptamine followed by 2, 3-dihydroxybiphenyl dioxygenase.