DOI: 10.36347/sajb.2014.v02i03.009

Pages: 214-223

Reactive oxygen species and free radicals are involved in the nephrotoxicity induced by a synthetic anticancer drug Cisplatin (CDDP). This paper is based on nonparticipation technique for preparation of Curcumin nanoparticles (CURNPs) and study the effect of Curcumin (CUR) and CURNPs (60, 30 mg/kg b.w) as antioxidants on the nephrotoxicity induced by CDDP. Physicochemical characterization was studied by using Dynamic Light Scattering (DLS) using Zetasizer Nano ZS, FTIR and X-ray diffraction. CUR and CURNPs were evaluated using CDDP (6mg/kg b.wi.p) induced oxidative renal damage in male rats. Urea and creatinine in serum were estimated for the renal function. Also, TBARS was estimated in serum. The results indicated that CUR and CURNPs (60, 30 mg/kg b.w) significantly (p<0.05) protected the nephrotoxicity induced by CDDP. The CDDP induced increase of urea and creatinine concentrations were reduced in the CUR (60, 30 mg/kg b.w) + CDDP and CURNPs (60, 30 mg/kg b.w) + CDDP treated groups. On the other hand, CDDP induced rise of serum TBARS. TBARS, was decreased significantly (p<0.05) in groups treated by CUR and CURNPs (60, 30 mg/kg b.w) and protected from the increase of CDDP induced lipid peroxidation. This study concluded that CUR and CURNPs (60, 30 mg/kg b.w) are effective to protect against oxidative renal damage. Most importantly, CURNPs was much more effective and better nephroprotective agent than CUR. Hence, CURNPs has a strong potential to be used as a strong antioxidant in CDDP nephrotoxicity.