DOI: 10.36347/sajb.2014.v02i11.015

Pages: 822-824

Desire to leave genetic footprints has been a long cherished dream of mankind. The concept of sperm banking has fulfilled these desires by preserving gametes and genital tissues. Cryo banking of spermatozoa is an essential aspect of fertility preservation. Developments in the field of cryopreservation have proved that frozen sperm is as good as fresh sperm in fertilizing oocytes. Semen banking has major role in fertility preservation in cancer patients. In the present study 94 samples were taken and evaluated with two different cryopreservation protocol. One method was to mix the pellet with the media and the other was the pellet mixed with the seminal plasma. From the above two adopted protocols the pellet mixed with the seminal plasma showed 55- 40% of sperm retrieval after thawing which was higher than the percentage obtained by the other method.