DOI: 10.36347/sjams.2016.v04i02.029

Pages: 451-459

Amoebiasis is one of important health problems in developing countries. It is commonly reported among human beings with different frequency of distribution in different parts of the world. The aim of this study was to detect clinical signs and symptoms and to compare sensitivity and specificity of conventional stool examination and qualitative enzyme immunoassay (EIA) panel kit to detect E. histolytica/ E. dispar among children in Kirkuk hospitals. The method in a study was carried on 800 stool samples from children attended Kirkuk hospitals for period from February 2007 to end of January 2009, as well as another 100 children samples of the near ages of the patients as a control group. The ages of children were ranging from below one month to 12 years old. The stool samples were examined by direct stool examination and qualitative enzyme immunoassay (EIA) panel kit. In results the morphology of E. histolytica cysts for which modified D’antoni’s iodine and 1% lugol’s iodine (weak iodine solution) was clearer than that strong iodine solution. It was found that the sensitivity, specificity, and efficiency of Enzyme immunoassay (EIA) test for E. histolytica/E. dispar were 91.07%, 98% and 93.2% respectively. The clinical signs and symptoms were increased when the bacterial infections associated with E. histolytica/E. dispar infections.