DOI: 10.36347/sjams.2016.v04i03.049

Pages: 872-876

Non-Fermenting Gram-Negative Bacilli (NFGNB )group includes numerous organisms but the ones which are known to cause nosocomial infections are Pseudomonas aeruginosa, Acinetobacter baumanii, Burkholderia cepacia complex (BCC) and Stenotrophomonas maltophila .This study was undertaken to identify the various nonfermenters isolated from patients admitted to our hospital, a tertiary care center, at Jammu. A total of 4585 clinical specimens were received in the laboratory, which included 1234 urine, 742 pus, and 1864 blood cultures, collected in a brain-heart infusion broth, 110 endotracheal catheter tips, 529 CSF samples and 115 body fluids. These samples were placed on blood agar, chocolate agar, and Mac Conkey's agar, and incubated at 37°C for 18-24 hours. The clinical isolates were identified using the conventional biochemical tests as per the standardized protocols. Non-fermenting Gram-negative bacilli were isolated from 572 out of 4585 clinical specimens accounting for an isolation rate of 12.40%. Pseudomonas aeruginosa was the most common isolate, accounting for 312 (54.54%), followed by Acinetobacter baumanii 235 (41.08%), Stenotrophomonas maltophilia 15 (2.62%), and Burkholderia cepacia complex 10 (1.70%). In our study, a total of 13 (86.66%) of the isolates were sensitive to Colistin and a total of 4 (26.60%) were sensitive to Imipenem. Thus NFGNB are emerging as important opportunistic pathogens and are resistant to commonly used antimicrobials. Therefore early diagnosis and institution of empirical therapy based on local antibiogram data of the institute would reduce mortality and improve patient management.