DOI: 10.36347/sajb.2015.v03i02.009

Pages: 166-169

P. recurvatum has been a very lead candidate for the discovery of novel antibiotics. The fungi was isolated from cocoa farm soil in Eri-pose, Ondo State Nigeria. Ethyl acetate extract was obtained after the culturing, extraction and purification of the cultured collected from a locally fabricated fermenter. The extract was fractionated through column chromatography using a mixture of solvent. The B4 fraction was screened for its antimicrobial efficacy. When tested against B. substilis, the zone of inhibition after 24hrs of incubation was 13.00 mm, also, screened against E. coli, the zone of inhibition was 11.00mm. The antibacterial activity of fraction was better than that of streptomycin standard when screened against B. substilis, its zone of inhibition was 12.00mm and had the same zones of inhibition with the fraction when screened against E. coli The results of the GC-MS revealed presence of major novel compounds which includes Furan, 4,5-diethyl-2,3-dihydro-2,3-dimethyl, 5-ethyl-5-methyl having retention time 5.20 and % total of 0.96, 2-phenyl-2-oxazoline having retention time 5.71 and % total of 2.14, 1,3,5-triazine,2,4 (1H,3H)-dione,6 ethylamino) with retention time 11.50 and % total of 1.36, Hydrocinnamic acid-0-[1,2,3,4-tetrahydro-2- naphthyl) methyl] having retention time 7.44 and % total 1.24. These major bioactive compounds have been found very useful in the formulation of novel drugs as they possess various pharmacological activities.