DOI: 10.36347/sajb.2015.v03i03.003

Pages: 248-253

High resolution proton nuclear magnetic resonance (NMR) spectroscopy has proved to be one of the most powerful technologies for producing a comprehensive profile of metabolite signals. Metabonomics is successful because disease, drugs or toxins cause perturbations of the concentrations and fluxes of endogenous metabolites involved in cellular pathways. The metabolic profile of urine, in particular, often shows changes in response to toxic or disease-induced stress, because of the system’s attempt to maintain homeostasis. In the present study, we aimed to explore the protective effect of DHA against aluminum (Al) induced nephrotoxicity and metabonomic changes in rats using histopathology and proton Nuclear Magnetic Resonance. The study highlights significant decrease the concentration of citrate, creatinine, allantoin, trans-aconitate succinate and increased acetate level in urine profile of experimental rats when compared with control rats. The docosahexaenoic acid (DHA) found to be reverses these metabonomic and cellular changes. These modulations may lead to identification of possible therapeutic intervention for Al induced nephrotoxicity, dialysis dementia and Alzheimer disease (AD).