DOI: 10.36347/sajb.2015.v03i03.004

Pages: 254-262

This study focus on the phytotoxicity of TiO2 nanoparticles (NPs) compared with bulk TiO2 particles B on: germination parameters, vegetative traits, roots viability, Biomass of seedling and photosynthetic pigments of Amber 33 variety of Rice (Oryza Sativa) in vitro. There were induction in seed germination percentage using different concentrations of B particles, while it decreased when the seeds exposed to NPs at concentrations (10, 1 and 0.01) mg/ml compared with control. There were differences between the effects of NPs compared with B particles on germination percentage. The highest germination rate had seen at (0.1 and 0.01) mg/ml concentration of NPs. The same features had seen at (1 and 10) mg/ml of B particles. Other concentrations were reduce the germination rate. All concentrations of NPs were reduced mean germination time. The same reduction has seen at (1-10) mg/ml of B particles. The lower concentrations (0.1-0.01) mg/ml of it were increased. The concentrations of B particles (10, 1 and 0.1) mg/ml showed increased in mean daily germination, whereas higher concentrations of NPs (10 and 1) mg/ml decreased it. However slight increase at 0.1 mg/ml and 0.01 mg/ml of it in MDG was observed. All concentrations of B particles were increase: vigor index I, vigor index II, germination value and promoter indicator compared with control. There were different changing in vigor index I in dose of NPs depending manner. There were differences between the effects of NPs (0.01, 1 and 10) mg/ml compared with B particles on vigor index I, vigor index II and promoter indicator. B particles and NPs were not effect on: shoots, roots, hairy roots length and total of plant lengths. While there were induction in number of hairy roots using 0.01 mg/ml concentration of NPs. NPs (10 and 1) mg/ml concentrations were reduced the number of hairy roots compared with control. The effect of these concentrations were different from the effect of the same concentrations of B particles.