DOI: 10.36347/sjams.2016.v04i06.079

Pages: 2252-2255

Tuberculosis (TB) is one of the commonest opportunistic infection and the leading cause of death in HIV patients in developing countries. HIV infection is a well recognised risk factor for both activation of initial infection and reactivation of latent infection. This study was done to find out the co-prevalence and the trend of HIV infection among tuberculosis patients and to determine the prevalence of MDR Tuberculosis in HIV positive patients using Xpert MTB/RIF assay (Cepheid, Sunnyvale, CA, United States).The sputum samples are received from patients attending ICTC clinic at Osmania General Hospital, Hyderabad. Sputum specimen was processed for TB detection by Cartridge Based Nucleic Acid Amplification testing (CB-NAAT) using Xpert MTB/RiF assay technology. A total of 125 HIV patients were in the study. Out of these patients 20 MTB cases were detected. Of these 20 cases 18(90%) cases were sensitive to Rifampicin (RIF) and 2(10%) cases were showing resistance to Rifampicin (RIF) drug. Co-existence of HIV and tuberculosis is high and there is high Prevalence of MDR tuberculosis in HIV patients. The Gene Xpert MTB/RIF assay is a novel integrated diagnostic device that performs sample processing and real-time PCR analysis in a single hands-free step for the diagnosis of tuberculosis and rapid detection of RIF resistance in clinical specimens.