The development of reliable and eco-friendly organisms of silver nanomaterials is an important aspect of current nanotechnology research and application. In current study, we report extracellular biosynthesis of metallic silver nanoparticles at two molar concentrations (1mM and 5mM) of AgNO3 using Bacillus sp. isolated from solid waste soil sample. The potential culture was isolated on nutrient agar medium and identified by several staining and biochemical tests. The potential isolate capable of reducing AgNO3 was identified as Bacillus sp. The reduction of AgNO3 was monitored by visible colour change of the solution. UV-Vis spectrum of silver nanoparticles (1mM and 5mM) obtained from the above bacteria containing silver ions showed a peak around 393nm and 405nm respectively. These nanoparticles (1mM and 5mM) were processed for antimicrobial activity and analyzed for plastic degradation. 5mM concentration of silver nanoparticles showed better results against Pseudomonas sp., Staphylococcus sp., and Aspergillus niger compared to 1mM concentration. Silver nanoparticles showed good antibacterial effect against Klebsiella sp. at 1mM concentration. Whereas, Candida albicans was found to be resistant to silver nanoparticles. Efficacy of the silver nanoparticles in degradation of plastics was analyzed by liquid culture method. Silver nanoparticles and bacteria degrade 18% of plastics and silver nitrate 0% of plastics after 15 days period;

A simple, rapid, economical, precise and accurate Stability indicating RP-HPLC method for simultaneous estimation of Diloxanide Furoate and Ornidazole in Their Combined Dosage Form has been developed. The separation was achieved by LC- 20 AT C18 (250mm x 4.6 mm x 2.6 μm) column and Buffer (pH 4.5): Acetonitrile (40:60) as mobile phase, at a flow rate of 1 ml/min. Detection was carried out at 277 nm. Retention time of Ornidazole and Diloxanide Furoate were found to be 4.620 min and 7.633 min, respectively. The method has been validated for linearity, accuracy and precision. Linearity observed for Ornidazole 5-15 μg/ml and for Diloxanide Furoate 7.5-22.5 μg/ml. The percentage recoveries obtained for Ornidazole and Diloxanide Furoate were found to be in range of 100.88 ± 0.60 and 100.85± 0.20 respectively. The proposed method was successfully applied for the simultaneous estimation of both the drugs in commercial combined dosage form

The hypersplénismes correspond to a syndrome characterized by the presence of splenomegaly and pancytopenia predominantly platelet lineage. It is associated with disorders of hemostasis. We conducted a prospective study on a year May 2014 to April 2015. It was conducted at the department of anesthesia resuscitation CHU Le Dantec. We included all patients undergoing hypersplenism. During the study period 4 patients were operated. There are two men and two women aged 35 and 67 years respectively, 34 and 20 years. We found lymphoma in a case and cirrhosis in the other three cases. Severe thrombocytopenia associated with hemostasis disorders was found in all cases. A transfusion of platelet concentrates, packed red blood cells and fresh frozen plasma was made to prepare for the intervention. All underwent general anesthesia. Hemorrhagic shock was noted in all patients. The postoperative complications were simple without postoperative noted. Hypersplenism associated with hemostasis disorders poses anesthetic management problems. All patients had significant hemostatic disorders associated with severe thrombocytopenia despite the correction preoperatively. Anesthesia of these patients imposes support measures hemorrhagic shock