Biosynthesis of silver nanoparticles using Bacillus sp. for Microbial Disease Control: An in-vitro and in-silico approach
Shivani Tiwari, Jyotsna Gade, Anjali Chourasia, Aruna J, Geetha. GR, Kumar.KM
Sch Acad J Pharm | 389-397
DOI : 10.36347/sajp
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The development of reliable and eco-friendly organisms of silver nanomaterials is an important aspect of
current nanotechnology research and application. In current study, we report extracellular biosynthesis of metallic silver
nanoparticles at two molar concentrations (1mM and 5mM) of AgNO3 using Bacillus sp. isolated from solid waste soil
sample. The potential culture was isolated on nutrient agar medium and identified by several staining and biochemical
tests. The potential isolate capable of reducing AgNO3 was identified as Bacillus sp. The reduction of AgNO3 was
monitored by visible colour change of the solution. UV-Vis spectrum of silver nanoparticles (1mM and 5mM) obtained
from the above bacteria containing silver ions showed a peak around 393nm and 405nm respectively. These
nanoparticles (1mM and 5mM) were processed for antimicrobial activity and analyzed for plastic degradation. 5mM
concentration of silver nanoparticles showed better results against Pseudomonas sp., Staphylococcus sp., and Aspergillus
niger compared to 1mM concentration. Silver nanoparticles showed good antibacterial effect against Klebsiella sp. at
1mM concentration. Whereas, Candida albicans was found to be resistant to silver nanoparticles. Efficacy of the silver
nanoparticles in degradation of plastics was analyzed by liquid culture method. Silver nanoparticles and bacteria degrade
18% of plastics and silver nitrate 0% of plastics after 15 days period;
Analytical method development and validation of diloxanide furoate and ornidazole in its combined pharmaceutical dosage form
Hemangi Vaidya, Sweetu Patel, Divya Patel, Prasanna K Pradhan, Umesh Upadhyay
Sch Acad J Pharm | 398-404
DOI : 10.36347/sajp
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A simple, rapid, economical, precise and accurate Stability indicating RP-HPLC method for simultaneous
estimation of Diloxanide Furoate and Ornidazole in Their Combined Dosage Form has been developed. The separation
was achieved by LC- 20 AT C18 (250mm x 4.6 mm x 2.6 μm) column and Buffer (pH 4.5): Acetonitrile (40:60) as
mobile phase, at a flow rate of 1 ml/min. Detection was carried out at 277 nm. Retention time of Ornidazole and
Diloxanide Furoate were found to be 4.620 min and 7.633 min, respectively. The method has been validated for linearity,
accuracy and precision. Linearity observed for Ornidazole 5-15 μg/ml and for Diloxanide Furoate 7.5-22.5 μg/ml. The
percentage recoveries obtained for Ornidazole and Diloxanide Furoate were found to be in range of 100.88 ± 0.60 and
100.85± 0.20 respectively. The proposed method was successfully applied for the simultaneous estimation of both the
drugs in commercial combined dosage form
Getting anesthetic management of splenectomy for hypersplenism at the University Hospital of the Aristide Le Dantec hospital
Fall ML, Barboza D, Diaw B Gaye I, Ba B, N Diop, Farid E, E. Diouf
Sch Acad J Pharm | 405-407
DOI : 10.36347/sajp
Abstract
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The hypersplénismes correspond to a syndrome characterized by the presence of splenomegaly and
pancytopenia predominantly platelet lineage. It is associated with disorders of hemostasis. We conducted a prospective
study on a year May 2014 to April 2015. It was conducted at the department of anesthesia resuscitation CHU Le Dantec.
We included all patients undergoing hypersplenism. During the study period 4 patients were operated. There are two men
and two women aged 35 and 67 years respectively, 34 and 20 years. We found lymphoma in a case and cirrhosis in the
other three cases. Severe thrombocytopenia associated with hemostasis disorders was found in all cases. A transfusion of
platelet concentrates, packed red blood cells and fresh frozen plasma was made to prepare for the intervention. All
underwent general anesthesia. Hemorrhagic shock was noted in all patients. The postoperative complications were simple
without postoperative noted. Hypersplenism associated with hemostasis disorders poses anesthetic management
problems. All patients had significant hemostatic disorders associated with severe thrombocytopenia despite the
correction preoperatively. Anesthesia of these patients imposes support measures hemorrhagic shock