A simple and selective LC method is described for the determination of ASENAPINE dosage forms. Chromatographic separation was achieved on a C18 column using mobile phase consisting of a mixture of Triethylamine Buffer: Acetonitrile (50:50) with detection of 220nm. Linearity was observed in the range 15-45 µg /ml for ASENAPINE (r2 =0.997) for the amount of drug estimated by the proposed methods was in good agreement with the label claim. The proposed methods were validated. The accuracy of the methods was assessed by recovery studies at three different levels. Recovery experiments indicated the absence of interference from commonly encountered pharmaceutical additives. The method was found to be precise as indicated by the repeatability analysis, showing %RSD less than 2. All statistical data proves validity of the methods and can be used for routine analysis of pharmaceutical dosage form.

To evaluate the pharmacological action of leaf extract of Terminalia bellerica with Moringa oleifera for its synergistic action on anti-anxiety activity in rats. The pharmacological evaluation of standardized aqueous extract of the leaves of Terminalia bellerica with Moringa oleifera was carried by using the following Stimuli model: Antianxiety activity by Elevated plus Maze. The parameters of various activities to assess anti-anxiety were evaluated and found That, The combination of extracts possess a better response then alone. The aqueous leaf extract of the combination of Terminalia Bellerica with Moringa oleifera has shown significant anti-anxiety activity when compared with standard and extract alone.

When taking oral medications, it is recommended to take them with cold or warm water. However, if medication is taken regularly and the timing of ingestion is after meals, there are likely many cases in which the medicine was taken with fruit juice, tea, coffee, non-alcoholic drinks, and alcoholic drinks. The contents of non-alcoholic beverages are usually hidden and protected by law; therefore, unknown substances may impact the proliferation and function of gastrointestinal epithelial cells. The highestselling non-alcoholic drink is Coca-Cola (2017), and its sales are third in Japan (2017). Therefore, drug intake by Coca-Cola is considered to be greater than that by other nonalcoholic drinks. In this study, to clarify the influence of Coca-Cola on gastrointestinal epithelial cells, we examined the influence of Coca-Cola on the proliferation and cellular uptake of HGC-27, IEC-6 and Caco-2, which are gastrointestinal epithelial cell lines. In the limited conditions (Concentration of Coca-Cola: 4%, Exposure time of Coca-Cola: 6 hr), significant changes in the proliferation of human gastrointestinal epithelial cells or uptake were not observed.