Original Research Article
Oct. 26, 2020
Genetic Diversity of Growth Hormone Genes and Its Relationship with Weight Coast Gain of Pesisir Cattle
Mangku Mundana, Sarbaini, Yurnalis, Jaswandi
Sch J Agric Vet Sci | 219-230
DOI : 10.36347/sjavs.2020.v07i10.001
This study aimed to: 1) Obtain diversity of body weight gain in Coastal cows, 2) Obtain diversity of growth hormone genes (GH) in intron 3 to exon 4 (GH4) and intron 4 to exon 5 (GH5) in Coastal cattle using the polymerase method chain reaction-restriction fragment length polymorphisms (PCR-RFLP), 3) Obtain a relationship between diversity of growth hormone genes (GH4 and GH5) with body weight gain in Coastal cows. This research was conducted in the field and in the laboratory. Field research was conducted to obtain data on body weight gain and blood samples from Coastal cows. Samples used amounted to 141 Coastal cows with age around 1-4 years where Coastal cows came from Ranah Pesisir District and Bayang District in Pesisir Selatan Regency, West Sumatra Laboratory research was conducted at the Laboratory of Animal Biotechnology, Faculty of Animal Husbandry, Andalas University. The diversity of the GH gene was determined by gene frequency, heterosigosity values, the value of Polymorphic Informative Content (PIC), gene balance in the population, and the relationship between the diversity of GH genes with body weight gain in Coastal cattle. The results of the field research showed that the average body weight of Male Coastal cattle was 0.142 ± 0.086 kg or 142 gr / cow / day with a high coefficient of diversity that was 20.4% and on CoastalFemale cows 0.195 ± 0.153 kg or 195 gr / cow / day with a coefficient diversity of 73.3%. Laboratory research results obtained the diversity of GH (polymorphic) genes in the GH4 | AluI gene and the GH5 | Eco471 gene. Genotype frequency of GH4 gene fragments Gene AluI is AA of 0.79 and BB of 0.21 and allele frequencies (A) and (B) for GH4 | AluI are 0.79 and 0.21, and for GH5 | Eco471 genotype frequencies are TT, TC, and CC respectively according to 0.52, 0.34, and 0.14 while the allele frequencies were T alleles 0.69 and C 0.31. The observed Heterozygosity values and expectations of GH4 | AluI and GH5Eco471 were 0.00, 0.34 and ......