Original Research Article
April 15, 2021
Cloning, Bioinformatics and Activity Analysis of the MnhB Gene from the Moderately Halophilic Bacterium Halobacillus Y5
Wang Yongjie, Liu Haoran, Zhang Guannan, Zhu Lingli, Yuan Shuai, Wang Yanhong
Sch J Eng Tech | 40-45
DOI : 10.36347/sjet.2021.v09i04.001
Objective: The mnhB gene of Halobacillus Y5 was cloned to clarify its basic biological information. Methods: The genome of Halobacillus Y5 was extracted and the gene fragments were obtained by PCR, then 400~900 bp fragments were recovered and linked to vector pUC18. The constructed recombinant plasmids were transformed to competent E.coli KNabc by functional complementation, and the strain containing the gene of Na+/H+ antiporters was screened for bioinformatics analysis. The positive monoclonal strains were screened by specific medium. Results: mnhB gene has two CDS regions, respectively 305 bp and 128 bp, encoding 96 amino acids. The predicted relative molecular weight is 11048.16 Dt, the isoelectric point is 4.66, the fat coefficient is 130.40, and the instability coefficient is 29.15. It is a stable protein with a half-life of 30 h. Comparison of similarity between mnhB from Halobacillus Y5 and 5 sequences, the homology of wp_101843976.1, wp_082234107.1, wp_079529991.1, wp_128522668.1, wp_08502981.1 were 93%, 69%, 78%, 69%, 67%, respectively. Through phylogenetic tree analysis, the Na+/H+ antiporters from Halobacillus Y5 is in a separate branch, which may be a new member of Na+/H+ antiporter. Na+(Li+, K)/H+ antiport activity was detected from everted membrane vesicles prepared from E. coli /pUC- mnhB but not those of /pUC18. Conclusion: A new mnhB gene was successfully cloned, which laid a theoretical foundation for the development and utilization of Halobacillus Y5.
Original Research Article
April 29, 2021
Information and Communication Technology Word processing skills’ In-Service Training for Computer Teachers in Secondary Schools in Nsukka Education Zone
Nwosu Ogochukwu F, Osondu Stella Ifeoma E, Nwanze Maureen N
Sch J Eng Tech | 46-50
DOI : 10.36347/sjet.2021.v09i04.002
This paper focuses on the computer studies teachers ICT word processing skills of secondary school computer studies teachers in Nsukka education zone, Enugu state, Nigeria. The purpose of this study is to find out the ICT word processing skills needed by computer studies teachers in secondary schools in Nsukka education zone, Enugu state Nigeria. 120 copies of questionnaire were administered to computer studies teachers in 58 secondary schools, of which all were completed and returned. The findings of this study showed that computer studies teachers in secondary schools in Nsukka education zone needed in-service training in word processing skills which include: ability to create blank document from templates and from existing document, ability to cut, copy, paste text, move text by dragging and dropping, among others. The study concluded that secondary schools computer studies teachers needed in-service training in word processing skills for effective teaching and learning of computer studies in secondary schools in Nsukka educational zone. That the identified word processing skills should be used by curriculum planners at State and federal levels to further improve the existing curriculums at secondary in the State and the nation in general.