DOI: 10.36347/sjams.2018.v06i01.056

Pages: 272-277

Hemolysis is one of the most common reason for inadvertent reports, and sample rejection in clinical laboratory. The aim of this study is to know the effect of hemolysis on routine biochemical parameters. Fifteen healthy volunteers are selected for this study. Venous plasma is collected and grouped into four levels of hemolysis based on hemoglobin concentration : Group I: 0-0.10 g/L, Group II: 0.10-0.50 g/L, Group III: 0.51-1.00 g/L, Group IV: 1.01-2.50 g/L, Group V: 2.51-4.50 g/L. Lysis is achieved by mechanical agitation. The analytes are measured in Automated Biochemistry Analyser. Aspartate aminotransferase (AST) levels are significantly affected even at undetectable levels of hemolysis.Variations of potassium and total bilirubin are observed in moderately hemolyzed samples (hemoglobin >1 g/L). Alanine aminotransferase (ALT), Cholesterol, and Inorganic phosphate (Pi) concentrations are not interfered up to severely hemolyzed levels (hemoglobin: 2.5-4.5 g/ L). Albumin, Alkaline phosphatase (ALP), Amylase, Chloride, HDL-cholesterol, Creatine kinase (CK), Glucose, Total protein, Triglycerides, and Uric acid levels vary significantly but within allowable limits. Hemolysis causes alteration of many parameters in Biochemistry. It is therefore preferable to do hemoglobin estimation in plasma for analytes with potential interference, to avoid wrong reporting of results.