DOI: 10.36347/sjams.2023.v11i11.004

Pages: 1881-1885

Background: The spread of antibiotic-resistance genes in ecosystems has led to the emergence of antibiotic- resistant bacteria, leading to various antibiotic-resistant diseases worldwide. Materials and methods: This study aimed to characterize mute-drug resistant Staphylococci isolates from urine and wounds. We obtained 400 bacterial isolates that were tested for their pathogenicity through cultivation on blood agar, Vitek instrument was used for characterization of different antibiotic sensitivity, while PCR was used to detect the resistance gene. Excel and SPSS were used to analyze the data. All samples were obtained after institutional ethics review and participant's-consents Result: Of 400 160=40% females, 60%=240 were males) isolates were carried out from. Out of 400 twenty-one were beta-hemolysis almost of them were described as extensive Multi-drug resistant isolates were 4(21) as they resist 10 antibiotics, 1(21) resist nine antibiotics, 7(21) resist eight antibiotics, 3(21) against seven, 2(21) six antibiotics, 2(21) resist five antibiotics,1(21) resist four antibiotic and 1(21) resist two antibiotics. These twenty-one were positive in mecA gene amplification through PCR technique but negative for the Vancomycin resistance gene. Conclusion: There are high mecA genes among clinical isolates in our study further studies with a high sample size Staphylococcus haemolyticous is recommended.