Antihypertensive effect of Telmisartan is result of it’s action by specific blockage of angiotensin II receptors. The aim of current study was the application of the validated UV-spectrophotometric method for determination of Telmisartan at λ = 298 nm in 99.98 % ethanol. UV-VIS diode array spectrophotometer was used. From the homogenized tablets of Telmisartan tabl. 80 mg accurately were measured samples, containing an amount, equivalent to 80 mg Telmisartan and were dissolved to 100.0 ml with 99.98 % ethanol in volumetric flasks. From the obtained solutions, an aliquot parts of 1.0 ml were diluted separatelly with the same solvent to 100.0 ml. Data for Chauvenet’s criterion are lower than maximum permissible value (U = 1.73; N = 6), which was applied for the assessment of the need for the removal of sharply different results. Analytical parameter precision was proved by the fact, that all results for the quantities in model mixtures and in tablets correspond to the relevant confidence interval: model mixtures: 80.06 mg  81.34 mg; tablets 80 mg: 77.79 mg  81.09 mg. Standard deviations were lower than 1.2; related standard deviations were lower than 1.6 % and relative errors were lower than 0.7 %. The validated method can be applied for the determination of Telmisartan in dosage drug preparations.

The study aimed to investigate the wound healing activity of the seeds of Dolichos lablab, Phaseolus lunatus, and Glycine soja. In the present study the aqueous extracts of the seeds were orally administered to the Sprague Dawley rats. Evaluation of wound healing activity was done using the incision and excision wound models. In each model, group I served as the control group, group II as the test group treated with Dolichos lablab extract, group III as the group treated with Phaseolus lunatus extract and group IV with Glycine soja extract. Incision wound model rats were treated for 10 days and the wound healing activity was assessed by the tensile strength and histopathological studies of the granulation tissue. Excision wound model rats were treated until the wounds were completely closed. In this model, wound healing activity was assessed by the rate of wound closure and free radical scavenging activity was assessed using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. In both models, hydroxyproline content in the scab was determined. The results of the study indicated that the extract of Dolichos lablab possessed better wound healing and antioxidant activity than Phaseolus lunatus and Glycine soja extracts.

The SLC22 transporter family, which belongs to the secondary active transporters, consists of many members that are expressed in the kidney, liver, small intestine and other tissues. Among them, organic cation transporter (OCT) 1 and OCT2, which transport many important substances, are physiologically important transporters. On the other hand, after organ transplantation, the transplanted organs (tissue) are exposed to ischemia/reperfusion (I/R) injury. Moreover, patients must undergo immunosuppressive therapy and are exposed to many drugs in many cases. Herein, we examined changes in mRNA expression levels of OCT1 and OCT2 in the rat small intestine during intestinal I/R. An intestinal I/R model was made by occluding the superior mesenteric artery and vein in rats using surgical-sutures and spring balance for 60 min, followed by reperfusion. In both the jejunum and ileum, a significant change in OCT1 and OCT2 mRNA expression was observed, and the changes in the ileum continued for at least 24 hr after reperfusion. The changes in expression level of mRNA differed between OCT1 and OCT2, as well as between the jejunum and ileum. Data on the changes in expression level of the transporters during intestinal I/R is very important from the viewpoint of drug therapy.